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. 2017 Aug 7;13(8):e1006561. doi: 10.1371/journal.ppat.1006561

Fig 6. Effect of ORFV073 on NEMO protein levels during ORFV infection.

Fig 6

(A) OFTu cells were mock infected (UN) or infected with OV-IA82 or OV-IA82Δ073 (MOI = 10) and harvested at 30 min, 45 min, 1 h, 1 h 30 min and 2 h p.i. Cytoplasmic extracts (50 μg) were resolved by SDS-PAGE, blotted to nitrocellulose membranes and probed with antibodies against NEMO and GAPDH. Densitometry of NEMO bands shown in B and C are normalized to the loading control GAPDH. (B) Fold changes are shown relative to uninfected control and results are mean values of two independent experiments (*, P<0.05). (C) Fold changes are shown relative to OV-IA82 and results are mean values of seven independent experiments (*, P<0.05)