Table 2. Epitope variants contained in plasmid and virus libraries.

Epitope	Codon Mutants	Possible	In Plasmid Library	In Virus Library	Replicating
SL9	Single	209	209	77	36.8%
	Double	19,855	8,505	75	0.88%
	Both	20,064	8,714	152	1.74%
KF11	Single	247	247	33	13.4%
	Double	28,158	10,754	18	0.17%
	Both	28,405	10,901	51	0.46%
KK10	Single	228	228	145	63.6%
	Double	23,826	9,557	3	0.03%
	Both	24,054	9,785	148	1.51%

The number of possible single and double amino acid variants within each library (excluding stop codons) is given, as well as the number of these variants detected above threshold in the plasmid library (≥ 2.5x10^-5, assumed to be adequate for representation after transfection of producer cells and passaging in 5 x 10⁶ cells thus yielding 20x sampling), and the number that carried forward above threshold (≥ 10⁻⁴ in both biological replicates, assumed to have replicated after transfection) in the virus libraries. Epitope variants below this frequency tended to decay if present in only one library, and thus were considered nonviable. If a variant was present above threshold in the plasmid library but inadequately represented in the virus library produced by transfection of the plasmid library into producer cells, it was also considered to be nonviable.