Table 1. Efficiency of FLP-mediated recombination.
| Promoter | Cell Typea | Stageb | Observedc | Expected | Efficiency (%) |
|---|---|---|---|---|---|
| dat-1 | Dopaminergic neurons | L2-adult | 8.0 ± 0.0 (15) | 8 | 100 |
| dpy-7 | Hypodermal (vulval cells) | L2 | 6.0 ± 0.0 (3) | 6d | 100 |
| L3 | 12.0 ± 0.0 (5) | 12d | 100 | ||
| L4 | 22.0 ± 0.0 (2) | 22d | 100 | ||
| elt-2 | Intestine | L1 | 20.2 ± 1.0 (14) | 20 (19–22) | 101 |
| L2 | 31.3 ± 1.1 (9) | 32 (30–34) | 98 | ||
| hlh-8 | M lineage | Adult | 19.2 ± 4.9 (18) | 32 | 60 |
| hsp-16.41 | Ubiquitous | L4–adult | Overlap with mCh::HIS-positive nuclei (20) | 92e | |
| lag-2 | Multiple (distal tip cell) | L3–adult | 1.0 ± 0.0 (8) | 1f | 100 |
| mec-7 | Mechanosensory neurons | L4–adult | > 12 (8) | 12 | NAg |
| myo-2 | Pharyngeal muscle | Adult | 33.5 ± 1.6 (14) | 37 | 91 |
| myo-3 | Body wall muscle | L1–L2 | 80.4 ± 1.3 (9) | 81 | 99 |
| nhr-82 | Seam cell lineage | Adult | 86.2 ± 22.2 (25) | 130 | 66 |
| rgef-1 | Neurons (dopaminergic and GABAergic) | L2–L4 | Overlap with unc-47 and dat-1 markers (15) | 99h | |
| tph-1 | Serotonin-producing neurons | Adult | 6.0 ± 0.0 (14) | 6 | 100 |
| unc-47 | GABAergic motor neurons | L2-adult | 19.2 ± 4.3 (27) | 26 | 74 |
Quantification of FLP D5 activity in different tissues using the dual color reporter (see Figure 1). For all strains, we observed multiple recombination events in all animals. mCh, mCherry; NA, not applicable; GABA, γ-aminobutyric acid.
Cell types where the promoter is active; cell types used for quantification of recombination efficiency are indicated in brackets.
Developmental stage that was used for determination of recombination efficiency; recombination was also observed in other stages.
Average number of GFP-positive nuclei ± SD; number in brackets: n.
Efficiencies were calculated based on recombination in central Pn.p cells and their descendants: recombination was also observed throughout the hypodermis.
Experiments performed with NLS-FLAG-FLP D5; recombination efficiency was calculated as percentage of nuclei coexpressing mCh::HIS-58 and GFP::HIS-58 after two rounds of heat induction (see Figure 4).
Efficiency was calculated based on recombination in the distal tip cell most proximal to the microscope objective; recombination was also observed in other cell types.
Not applicable; we did not attempt to identify and evaluate the 12 expected nuclei because expression levels were low and because we observed GFP in unexpected neurons.
Efficiency was calculated based on recombination in the dopaminergic neurons in the head and GABAergic motor neurons in the ventral nerve cord; recombination was also observed in other types of neurons.