Figure 4.

Viability of wild-type (WT) (SH3881) and rlm1Δ (SH4708 and SH4767) cell groups: (A) Viability of wild-type (magenta) and rlm1Δ (blue) cell groups grown under standard conditions on LA medium before sonication (left panel), after 10 pulses of sonication (center panel), and after 20 pulses of sonication (right panel). (B) Cell division of mother and bud cells after transfer from LA to YPDA medium. A typical wild-type and three typical rlm1Δ cell groups are shown at the indicated times after transfer. For the wild-type cell group shown in the left column and the rlm1Δ cell group shown in column (a), both the mother and daughter bud. For the rlm1Δ cell group in column (b), the mother cell buds but not the daughter cells. For the rlm1Δ cell group in column (c), neither the mother nor the daughters form buds. Bar, 10 μm. (C) Left panel: the percentage of mother cells that have budded by 5 hr for wild type (11/13) and rlm1Δ (9/16). Center panel: the percentage of buds that have budded by 5 hr for wild type (11/13) and rlm1Δ (2/49). Right panel: percentage of mother cells in which the nucleus stains with PI for wild-type and rlm1Δ strains, n = 4. (D) rlm1Δ satellite-daughter groups fixed and stained with PI. Bar, 5 μm. (E) Permeability: wild-type (magenta) and rlm1Δ (blue) strains were inoculated at 1 × 10⁶ cells/ml and grown for 7 hr at 30° in shaking cultures containing SLA medium (left), or these same strains were grown for 24 hr as colonies grown on LA medium (right). Cells from either growth condition were stained with trypan blue and examined by light microscope. As a positive control, wild type were incubated at 100° for 10 min (heat-killed, black). (F) Representative rlm1Δ satellite groups after 24 hr growth on LA medium stained with trypan blue. Bar, 10 μm.