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. 2017 Jun 1;206(4):2007–2039. doi: 10.1534/genetics.117.203174

Figure 12.

Figure 12

Oocyte M-phase entry is not required for expression of GFP::3xFLAG::MEG-1. The expression of GFP::3xFLAG::MEG-1 was examined by fluorescence and DIC microscopy in (A–H) mutant backgrounds or (I–L) after RNAi treatments as indicated. GFP::3xFLAG::MEG-1 expression is observed in small abnormal oocytes in the lin-41(tn1487ts) mutant background (strain DG4274) at both (A and B) 25° and (C and D) 22°. A spatial expansion of GFP::3xFLAG::MEG-1 expression is observed in proximal oocytes in unmated fog-2(oz40) females [(E and F); strain DG4251] and acy-4(ok1806) hermaphrodites [(G and H); from strain DG4260]. (K and L) GFP::3xFLAG::MEG-1 expression is observed after cdk-1(RNAi). Bar, 50 μm.