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. 2017 Jun 1;206(4):2007–2039. doi: 10.1534/genetics.117.203174

Figure 13.

Figure 13

LIN-41 represses expression of ORC-1. A functional mNG::3xFLAG::ORC-1 fusion protein was generated by CRISPR-Cas9 genome editing to examine protein localization in otherwise wild-type hermaphrodites; all strains shown contain the orc-1(tn1732[mng::3xflag::orc-1]) edit. (A) DIC and (B) fluorescence images of an adult hermaphrodite showing the expression of mNG::3xFLAG::ORC-1 in early embryos and the distal proliferative zone (pz). (C) DIC and (D) fluorescence images showing that mNG::3xFLAG::ORC-1 is first detectable in the embryo at the one-cell stage; arrowheads indicate mNG::3xFLAG::ORC-1 on mitotic chromatin [strain DG4228 is shown in (A–D)]. (E–J) mNG::3xFLAG::ORC-1 expression was detected by indirect immunofluoresence in dissected gonads stained with anti-FLAG antibody. DNA was detected with DAPI. Gonads from strain (E and F) DG4228, (G and H) DG4284, and (I and J) DG4293 are shown. (K) DIC and (L) fluorescence images of an orc-1(tn1732); lin-41(n2914); oma-1(zu405te33); oma-2(te51) (from strain DG4318) adult hermaphrodite showing the expression of mNG::3xFLAG::ORC-1 in most or all developing oocytes in addition to the distal proliferative zone. (A, B, and E–L) Bar, 50 μm. (C and D) Bar, 20 μm.