Genetic interaction between rte and rte2 mutants. (A) Phenotype of wild type, rte2-2, rte-1, and double rte-1;rte2-2 mutants grown in boron-deficient soil. (B and C) rte;rte2 double mutants are short in stature and showed rudimentary ear-like structures (arrowheads). (D) Longitudinal section of shoot tips from rte;rte2 double mutants stained with Toluidine blue, showing tissue anomalies (arrowhead) in the stem. (E) Longitudinal section of shoot stained with Safranin O-Alcian Blue. Lignified red patches (arrowhead) are visible. (F) Average plant height (n = 10, t-test, * P < 0.0001). (G) Average number of leaves (n = 10, t-test, * P < 0.0001). Error bars indicate SD. wt, wild type.