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. 2017 Jun 21;206(4):2053–2068. doi: 10.1534/genetics.117.202457

Figure 2.

Figure 2

Different effects of Mago and Srp54 inhibition on Ci-155 levels and activity. (A–C) Central region (around DV and AP borders) of wing discs expressing (A) UAS-Diap1, (B) UAS-mago RNAi, and (C) UAS-Srp54 RNAi plus UAS-Diap1 under the control of C765-GAL4, stained for ptc-lacZ (red) and Engrailed (En; green). Only Srp54 RNAi reduced ptc-lacZ expression and prevented Hh induction of anterior En (to the left of yellow arrows indicating the posterior edge of the AP border). (D and E) ptc-lacZ intensity profiles along the AP axis for (D) UAS-mago RNAi and (E) UAS-Srp54 RNAi compared to controls for a single experiment (average of 3–4 discs). (F) Maximal ptc-lacZ intensity as a percentage of controls for discs expressing mago and Srp54 RNAi, showing means and 95% C.I.s (n = 6 experiments for mago RNAi and n = 3 experiments for Srp54 RNAi). (G–J) Wing discs expressing (H) UAS-mago RNAi, (J) UAS-Srp54 RNAi, and (G and I) controls, stained for Ci-155 (white). (K–L) Ci-155 intensity profiles along the AP axis for single experiments (average of four wing discs), comparing (K) Mago and (L) Srp54 inhibition to controls. (M and N) Maximum Ci-155 intensity in anterior and AP border cells as a percentage of controls for discs expressing mago and Srp54 RNAi, showing means and 95% C.I.s (n = 5 experiments for mago RNAi and n = 4 experiments for Srp54 RNAi). AP, anterior/posterior; DV, ; Hh, Hedgehog; Ptc, Patched; RNAi, RNA interference; wt, wild-type.