Figure 7.

An intronless ci transgene tests ci RNA splicing as a key target of Mago and Srp54. (A–D and K–N) Ectopic ptc-lacZ (red) induced in anterior pka clones (marked by GFP, green, arrows) in wing discs null for ci with (A–D) one copy of a ci transgene and heterozygous for Su(fu) or (K–N) two copies of a ci transgene, was measured in the presence or absence of (A–D) mago RNAi or (K–N) Srp54 RNAi. (A, C, K, and M) ptc-lacZ expression was higher for discs with gCi than for SV-1 and was reduced by mago RNAi and Srp54 RNAi (A, B, K, and L) in the presence of gCi but (C, D, M, and N) not in the presence of SV-1. (I and S) ptc-lacZ expression in pka clones in discs of the designated genotypes relative to discs with gCi (and no RNAi), showing mean, SEM, and significant differences by Student’s t-test (* P < 0.05) for (I) n = 3, n = 3, n = 4, and n = 3 clones, and (S) n = 11, n = 9, n = 7, and n = 12 clones in the order shown. (E–H) Ci-155 (white) in ci null wing discs with two copies of gCi or SV-1 in the presence or absence of mago RNAi expression. (J) Maximal Ci-155 intensity as a percentage of wing discs with gCi, showing means and 95% C.I.s from two independent experiments of n = 6 discs for each condition. Ci-155 levels were reduced by Mago inhibition for gCi but not for SV-1. (O–R and T) ptc-lacZ (red) and Ci-155 (white) in ci null wing discs expressing UAS-Diap1 with two copies of gCi or SV-1 in the presence or absence of Srp54 RNAi. (T) Maximal ptc-lacZ intensity at the AP border as a percentage of wing discs with gCi, showing means and 95% C.I.s from two independent experiments of n = 6 discs for each condition. AP, anterior/posterior; RNAi, RNA interference.