Table 3. Functional annotation of ovary-specific, testis-specific, ovary–embryo coexpressed, and testis–embryo coexpressed genes for A. aegypti.
| Gene type | Role | P value |
|---|---|---|
| Ovary-specific | ||
| Cluster 1: enrichment score: 4.41 | Odorant binding | 6.50E−10 |
| Olfactory receptor activity | 4.10E−05 | |
| Plasma membrane | 5.10E−05 | |
| Olfaction | 5.90E−05 | |
| Olfactory receptor | 6.20E−05 | |
| Sensory transduction | 1.00E−04 | |
| Transducer | 1.40E−03 | |
| Receptor | 7.60E−03 | |
| Cluster 2: enrichment score: 2.08 | Integral component of membrane | 5.00E−04 |
| Transmembrane helix | 2.00E−02 | |
| Transmembrane | 2.00E−02 | |
| Membrane | 2.50E−02 | |
| Cluster 3: enrichment score: 1.24 | Sensory perception of taste | 1.90E−02 |
| 7TM chemoreceptor | 6.30E−02 | |
| Cell membrane | 1.60E−01 | |
| Testis-specific | ||
| Cluster 1: enrichment score: 2.36 | Leucine-rich repeat | 3.60E−04 |
| LRR_TYP (Leucine-rich repeats, typical) | 9.50E−03 | |
| Leucine-rich repeat, typical subtype | 2.40E−02 | |
| Cluster 2: enrichment score: 1.49 | Ves allergen | 1.60E−02 |
| SCP (SCP domain proteins) | 3.10E−02 | |
| Allergen V5/Tpx-1-related | 4.60E−02 | |
| CAP domain | 4.60E−02 | |
| Cluster 3: enrichment score: 1.24 | FBG (Fibrinogen-related domains) | 4.40E−02 |
| Fibrinogen, α/β/γ chain, C-terminal globular domain | 6.60E−02 | |
| Fibrinogen, α/β/γ chain, C-terminal globular, subdomain 1 | 6.60E−02 | |
| Ovary–embryo coexpressed (absent testis) | ||
| Cluster 1: enrichment score: 3.96 | Integral component of membrane | 2.90E−05 |
| Membrane | 1.60E−04 | |
| Transmembrane helix | 1.70E−04 | |
| Transmembrane | 1.80E−04 | |
| Cluster 2: enrichment score: 2.37 | Plasma membrane | 5.10E−05 |
| Odorant binding | 7.10E−05 | |
| Olfactory receptor activity | 1.10E−02 | |
| Olfactory receptor | 1.10E−02 | |
| Olfaction | 1.30E−02 | |
| Transducer | 1.60E−02 | |
| Sensory transduction | 2.10E−02 | |
| Receptor | 5.80E−02 | |
| Cluster 3: enrichment score: 1.96 | Gustatory receptor protein | 3.90E−03 |
| Gustatory receptor | 1.80E−02 | |
| Taste receptor activity | 1.80E−02 | |
| Testis–embryo coexpressed (absent ovary) | ||
| Cluster 1: enrichment score: 3.45 | Secreted | 7.90E−07 |
| Lipid metabolic process | 1.30E−05 | |
| Dol/Ves 1 allergen | 5.50E−04 | |
| Phosphatidylcholine 1-acylhydrolase activity | 6.30E−04 | |
| Lipase, N-terminal | 7.30E−04 | |
| Lipase | 7.30E−04 | |
| Carboxylic ester hydrolase activity | 4.00E−01 | |
| Cluster 2: enrichment score: 2.69 | ||
| Homeobox | 9.00E−05 | |
| Sequence-specific DNA binding | 9.40E−05 | |
| Homeodomain | 1.70E−04 | |
| HOX (Homeodomain and Homeodomain-like) | 2.60E−04 | |
| Homeobox, conserved site | 3.30E−04 | |
| Homeodomain, metazoa | 1.20E−03 | |
| DNA-binding | 1.80E−03 | |
| Homeodomain-like | 3.60E−03 | |
| Helix-turn-helix motif | 5.30E−03 | |
| Regulation of transcription, DNA-templated | 4.10E−02 | |
| Nucleus | 4.60E−02 | |
| Cluster 3: enrichment score: 1.92 | CAP domain | 1.00E−02 |
| Allergen V5/Tpx-1-related | 1.00E−02 | |
| SCP (SCP domain proteins) | 1.60E−02 |
The gene ontology was determined using the DAVID (database for annotation, visualization, and integrated discovery) system (Huang da et al. 2009), and the three clusters with the greatest enrichment score are shown per category. P-values are from a modified Fisher’s test, wherein lower values indicate greater enrichment. The number of genes per category is provided in Figure 4A.