Figure 3.

Inferred and observed steady state REPIN frequencies in P. fluorescens SBW25. (A) The observed frequencies of each mutation class are identical to the equilibrium frequencies for our quasispecies model at a mutation rate of $8.9\times {10}^{-11}$ (observed). We adjusted time to allow us to do simulations at a mutation rate of ${10}^{-4},$ i.e., each time step lasts long enough to allow for ∼10⁻⁴ mutations to occur for an underlying mutation rate of $8.9\times {10}^{-11}$ (adjusted quasispecies). The resulting adjusted quasispecies equilibria agree almost perfectly with the observed frequencies. A simulation of a simulation (10⁵ generations) with the same fitness values allows us to infer the variation of these frequencies (simulated). (B) Relative frequencies obtained from the simulation using the adjusted fitness values for ${10}^5$ generations. (C) Density plot of the relative frequencies of the mutation classes from our simulation.