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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Mech Ageing Dev. 2017 Jan 19;162:91–99. doi: 10.1016/j.mad.2017.01.004

Fig. 5.

Fig. 5

The adipocytes secrete chemokines which may recruit B cells to the VAT. A. Adipocytes were isolated from the VAT, sonicated for cell disruption in the presence of TRIzol to separate the soluble fraction (used for RNA isolation) from lipids and cell debris. Results show qPCR values (2−ΔΔCt) of CXCL10, CCL2, CCL5 RNA expression. Mean comparisons between groups were performed by Student's t-test (two-tailed). B. VAT B cells were isolated from the SVF and resuspended in TRIzol. Results show qPCR values (2−ΔΔCt) of CXCR2, CCR2, CCR3 RNA expression. Results in B are from those 3 mice among the 6 in A from which we had enough B cells. Mean comparisons between groups were performed by Student's t-test (two-tailed). *p<0.05, **p<0.01.