Figure 4.

Molecular disorganization of axons adjacent to microinfarct exists in the absence of significant changes in structural integrity of myelin and axons. Cortical tissue containing a microinfarct (A) stained for myelin basic protein (MBP, green) and neurofilament 200 (NF200, red) demonstrates very few demyelinated axons adjacent to the infarct core (B). Co-localization of MBP and NF200 indicates preservation of the structural organization of myelinated axons in regions both immediately adjacent to the infarct core (C) as well as some distance from the infarct core where disrupted axonal microdomain organization was observed (D). Inset: magnification ×60, scale bar = 5 µm.