Figure 7.

MAPK pathway regulated mIL-6Rα but not gp130 expression. iDCs were pretreated for 30 min with SB203580 (SB) or PD98059 (PD) 25 µM prior to lipopolysaccharide (LPS) maturation (50 ng/ml). Matured dendritic cells (DCs) were harvested at 24 and 48 h and analyzed by cytometry. (A) iDCs were treated as described above and mIL-6Rα expression was assessed (n ≥ 3). (B) iDCs were treated as described above and membrane gp130 expression was assessed (n ≥ 3). “*,” “**,” and “***” indicate statistical differences with, respectively, p < 0.05, p < 0.01, and p < 0.001.