Table 2.
Ranking of candidate reference genes according to stability values in a pool of Urochloa brizantha leaves samples subjected to various abiotic stresses.
| Gene Name | geNorm | NormFinder | BestKeeper | ΔCt | RefFinder | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rank order | Average expression stability (M) | Rank order | Stability Value (SV) | Rank order | Standard deviation [ + /−CP] | Rank order | Average of standard deviations | Rank order | Geometric mean of ranking values | ||||
| Heat Stress | |||||||||||||
| EF1-α | 6 | 1,297 | 1 | 0,727 | 5 | 1,21 | 1 | 1,35 | 1 | 2,34 | |||
| U2AF | 2 | 0,813 | 4 | 0,988 | 2 | 0,78 | 4 | 1,44 | 2 | 2,38 | |||
| β-TUB6 | 1 | 0,813 | 8 | 1,472 | 1 | 0,76 | 8 | 1,73 | 3 | 2,83 | |||
| Act12 | 5 | 1,231 | 2 | 0,788 | 6 | 1,46 | 2 | 1,39 | 4 | 3,31 | |||
| α-TUB5 | 3 | 0,976 | 5 | 1,009 | 3 | 1,12 | 5 | 1,47 | 5 | 3,87 | |||
| eIF4a | 7 | 1,374 | 3 | 0,943 | 8 | 2,02 | 3 | 1,41 | 6 | 4,74 | |||
| SucCoa | 4 | 1,109 | 6 | 1,018 | 4 | 1,19 | 6 | 1,49 | 7 | 4,90 | |||
| FTSH | 8 | 1,453 | 7 | 1,360 | 9 | 2,35 | 7 | 1,68 | 8 | 7,71 | |||
| UbiCo | 9 | 1,529 | 9 | 1,517 | 7 | 1,77 | 9 | 1,80 | 9 | 8,45 | |||
| Cold Stress | |||||||||||||
| EF1-α | 1 | 0,667 | 4 | 0,592 | 1 | 0,84 | 3 | 1,18 | 1 | 1,86 | |||
| UbiCo | 3 | 0,824 | 1 | 0,501 | 4 | 0,91 | 1 | 1,15 | 2 | 1,86 | |||
| α-TUB5 | 2 | 0,667 | 2 | 0,549 | 5 | 0,90 | 2 | 1,16 | 3 | 2,11 | |||
| eIF4a | 4 | 0,915 | 3 | 0,591 | 6 | 0,98 | 4 | 1,18 | 4 | 4,12 | |||
| SucCoa | 5 | 1,010 | 5 | 0,992 | 8 | 1,24 | 5 | 1,40 | 5 | 5,62 | |||
| Act12 | 7 | 1,109 | 8 | 1,360 | 2 | 0,87 | 8 | 1,55 | 6 | 5,66 | |||
| FTSH | 8 | 1,206 | 7 | 1,247 | 3 | 0,90 | 7 | 1,55 | 7 | 5,66 | |||
| β-TUB6 | 6 | 1,105 | 6 | 1,237 | 9 | 1,52 | 6 | 1,54 | 8 | 6,64 | |||
| U2AF | 9 | 1,392 | 9 | 1,496 | 7 | 1,00 | 9 | 1,73 | 9 | 8,45 | |||
| Drought Stress | |||||||||||||
| eIF4a | 1 | 0,565 | 1 | 0,656 | 6 | 0,96 | 1 | 1,26 | 1 | 1,57 | |||
| U2AF | 2 | 0,565 | 2 | 0,761 | 3 | 0,92 | 2 | 1,31 | 2 | 1,86 | |||
| α-TUB5 | 5 | 1,208 | 5 | 1,155 | 1 | 0,74 | 6 | 1,54 | 3 | 3,50 | |||
| SucCoa | 6 | 1,261 | 3 | 0,772 | 4 | 0,93 | 3 | 1,33 | 4 | 3,83 | |||
| β-TUB6 | 4 | 1,110 | 6 | 1,210 | 2 | 0,78 | 5 | 1,53 | 5 | 3,94 | |||
| EF1-α | 3 | 1,023 | 8 | 1,301 | 5 | 0,95 | 7 | 1,61 | 6 | 5,38 | |||
| Act12 | 7 | 1,324 | 4 | 0,867 | 7 | 1,03 | 4 | 1,36 | 7 | 5,29 | |||
| FTSH | 8 | 1,414 | 7 | 1,294 | 8 | 1,11 | 8 | 1,64 | 8 | 7,74 | |||
| UbiCo | 9 | 1,474 | 9 | 1,437 | 9 | 1,51 | 9 | 1,68 | 9 | 9,00 | |||