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. 2017 Aug 17;7:8627. doi: 10.1038/s41598-017-09059-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SEM-FIB (Dual–Beam) images of BV2 cells incubated with PAA-MNPs (50 μg/ml, ≈12 pg/cell) showing several clusters pinned at different points of the cell membrane (A,B and C). Cross-sectioning of one cell at one cluster location (D and E) showed clearly the insertion of the agglomerates through the cell membrane and penetration into the cytoplasm (C and D).