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. 2017 Aug 17;7:8627. doi: 10.1038/s41598-017-09059-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM images of BV2 cells containing 100 µg/ml of PAA-MNPs after 30 min of MHT at 46 °C. (A) vesicle-containing MNPs, ER: endoplasmatic reticulum, NE: nuclear envelope, MC: marginal chromatine, (D) dictyosome and M: mitochondrie. (B) MNPs cluster wrapped in lysosomal membrane (LM) and close to a residual body (RB). (C) Disrupted cell membrane near a large PAA-MNPs cluster after MHT. (D) Typical image of a completely damaged cell due to AMF and PAA-MNPs. (E) Cell with lysosome containing PAA-MNPs. Apoptotic cells (F and I) and dead cells (G and H) were found to coexist after MHT with (seemingly) alive cells containing high variable amounts of PAA-MNPs incorporated.