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. 2017 Aug 17;7:8596. doi: 10.1038/s41598-017-08674-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accuracy of microchip-based LAMP for detection of the MPL W515K/L mutation. (A) Agarose gel electrophoresis of the microchip-based LAMP-amplified products with the MPL W515 wild-type primer. MPL W515 wild-type plasmid (blue), MPL W515K mutant plasmid (orange), MPL W515L mutant plasmid (green), and negative control (gray). (B) Sequencing results of the LAMP-amplified products from A. The arrowheads indicate the location of the point mutations. The wild-type sequence is TGG at this location (MPL W515 wild-type plasmid). (C) Agarose gel electrophoresis of the microchip-based LAMP-amplified products from the MPL W515K mutant primer. MPL W515 wild-type plasmid (blue), MPL W515K mutant plasmid (orange), MPL W515L mutant plasmid (green), and negative control (gray). (D) Sequencing results of the LAMP-amplified products from C. The arrowheads indicate the location of the point mutation. The mutated sequence is AAG at this location (MPL W515L plasmid). (E) Agarose gel electrophoresis of the microchip-based LAMP-amplified products with the MPL W515L mutant primer. MPL W515 wild-type plasmid (blue), MPL W515K mutant plasmid (orange), MPL W515L mutant plasmid (green), and negative control (gray). (F) Sequencing results of the LAMP-amplified products from E. The arrowheads indicate the location of the point mutation. The mutated sequence is TTG at this location (MPL W515L plasmid).