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. 2017 Aug 17;7:8603. doi: 10.1038/s41598-017-09152-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Light-Sheet Fluorescent Imaging, Histogram Image Processing, and 3-D Reconstruction. (A) Collimated lasers are transmitted through an illumination lens (IL) to generate a light-sheet sectioning the sample. The detection arm includes an objective lens (DL) positioned orthogonally to the illumination path for fluorescence detection. The detection axis needs to exactly conjugate the illuminated plane with the camera CMOS plane. (B) We obtained the lateral and axial optical spatial resolution by measuring the point spread function (PSF) of 0.5 μm beads, expressed as the FWHM from the point source in the XY, XZ, and YZ planes. (C) Summary of the image reconstruction and 3-D rendering process in 3 phases encompassing input image, automated image segmentation, and 3-D reconstruction. BE: beam expander. CL: cylindrical lens. DC: dichroic mirror. DL: detection lens. FW: filter wheel. FWHM: full width at half maximum. IL: illumination lens. M: mirror. sCMOS: scientific complementary metal oxide semiconductor. SL: scan lens. TL: tube lens. Scale bar: 5 μm.