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. 2017 Aug 17;7:8614. doi: 10.1038/s41598-017-09258-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relaxation assay of TOP1MT, TOP1MT V256I variant, and TOP1 on negatively and positively supercoiled pBR322 DNA. Sc−: Negatively supercoiled pBR322 plasmid DNA; Sc+ : positively supercoiled pBR322 plasmid DNA; C, control, no enzyme added; the numbers on top of the figures are dilution factors of corresponding enzymes; 0, no dilution; 1, 1:5 dilution; 2, 1:25 (5²) dilution; 3, 1:125 (5³) dilution; 4, 1:625 (5⁴) dilution. Representative gels were shown.