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. 2017 Aug 17;7:8648. doi: 10.1038/s41598-017-08917-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ATPase activities of fusion proteins. (a) Schematic drawing of the construction of TK-B, TK_ΔSBDα-B, and TK_NBD-B are shown. (b) ATPase activities of TK-B, TK_ΔSBDα-B, TK_NBD-B and their mutants carrying K69A and T195A mutations in the TDnaK portion. ATPase activities in the absence (black bar) or presence of TGrpE (0.1 μM; gray bar, 1.0 μM; open bar) are shown. (c) ATPase activities of TClpB and TK-B and their mutants carrying E271Q and/or E668Q mutations in the TClpB portion are shown. (b,c) The measurements were performed at 55 °C in the presence of 3 mM ATP by using an ATP regeneration system. Error bars represent standard deviations of three or more independent measurements. WT, wild-type.