Figure 3.

van’t Hoff plots for the interaction of ^FIIRE-RNA with IRP1 repressor protein in the absence and presence of Mn²⁺. The data are indicated as FRT IRE-RNA (‒○‒), FRT IRE-RNA + 50 µM Mn²⁺ (‒⦁‒), ACO2 IRE-RNA (‒∆‒) and ACO2 IRE-RNA + 50 µM Mn²⁺ (‒▲‒) with IRP1 protein. Enthalpy and Entropy were determined from the slope and intercept of the temperature dependent equilibrium binding measurements, respectively.