Table 1.

Temperature dependent equilibrium dissociation constants (K _d) for the interaction of FRT IRE-RNA and ACO2 IRE-RNA with IRP1 protein in the absence and presence of 50 µM Mn²⁺.

Complex	K d (nM)
	5 °C	10 °C	15 °C	20 °C	25 °C	30 °C
FRT IRE-RNA/IRP1	4.6 ± 0.2	7.0 ± 0.2	8.6 ± 0.3	11.7 ± 0.4	14.2 ± 0.3	19.2 ± 0.4
FRT IRE-RNA-Mn2+/IRP1	83.3 ± 4	102 ± 6	112.4 ± 5	137.4 ± 6	174 ± 4	193 ± 5
ACO2 IRE-RNA/IRP1	55.9 ± 3	69.6 ± 5	89 ± 3	101 ± 7	129 ± 3.3	155 ± 4
ACO2 IRE-RNA-Mn2+/IRP1	535 ± 33	593 ± 29	648 ± 35	701 ± 31	750 ± 32	801 ± 36