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. 2017 Aug 17;7:8528. doi: 10.1038/s41598-017-09105-4

Figure 1.

Figure 1

Fabric and hybrid analysis. (A) Fabric architectures (1st and 5th columns) were first examined without biofilms and unfixed using scanning electron microscope (SEM, thick scale bars = 300 µm), and analyzed by FiberMetric (2nd and 6th columns showing fiber diameter distribution. Asterisks denote fabrics of various types which improved biofilm growth: *synthetic fabric made of polyester and cotton with large-sized threads and dense weave, **natural fabric made of cotton with small-sized threads and plain weave, ***synthetic fabric made of polyester with large fibres and loose weave. Fabric-biofilm hybrids were fixed after 3 days in culture and visualized in SEM (3rd and 7th columns, thin scale bars = 100 µm). Biofilm viability was scored each day on a score of 0–3 (0 = no growth and clear medium, 3 = full growth and cloudy medium, 4th and 8th columns). Control biofilm without fabric was scored 0–1–2–3. Results representative of 3 independent repeats. (B) Image analysis showing simulated roughness of biofilm in each of the three representative fabrics (*,**, ***, from left to right), demonstrating the correlation between weaving density and biofilm structure (x and y axes, location pixels; z axis, pixel intensity on a scale of 0–255).