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. 2017 Aug 17;7:8630. doi: 10.1038/s41598-017-09217-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The maturation of grafted cardiomyocytes is shown. (a–i) Representative images of sections stained with cardiac troponin I (cTnI) and β-myosin heavy chain (βMHC). Note that the antibody against βMHC reacts with human-derived graft cardiomyocytes but not with rat-derived host cardiomyocytes. (d–i) Magnified images of (a–c). While the sarcomere structure was easily observed in the grafts of the PSC/MG group, it was relatively rarely observed in the HyStem and PSC/HyStem groups. (j) % of cTnI-positive cells in all grafted cardiomyocytes, *P < 0.05. (k) Length of the sarcomere in grafted cardiomyocytes, **P < 0.01. PSC, pro-survival cocktail; MG, Matrigel.