Table 1.
Specific primers applied for the CfPAG-L amplifications with gDNA templates
| Forward primers | Reverse primers | ||
|---|---|---|---|
| Name | Sequence (5′–3′) | Name | Sequence (5′–3′) |
| ATG_F | ATGAAGTGGATAGTGGTGGCC | ||
| Exon2_F | TTCCTGAAGAMSCACAA | Exon2_R | TCATGGAGAACACTGAAGTC |
| Exon3_F | GCTCCTCCAACCTGTGGGT | Exon3_R | ACCCACAGGTTGGAGGAGCC |
| Exon4_F | ACCTACCACACCGATAAGAAGA | Exon4_R | TCTTCTTATCGGTGTGGTAGGT |
| Exon5_F | GATGGCATCMTGGGSCTGGCCTA | Exon5_R | TAGGCCAGSCCCAKGATGCCATC |
| Exon6_F | GTGTGGACAAGCGCCTGTACAA | Exon6_R | CTCCCCCATCCTTAGAGCCTTC |
| Exon7_F | TTGTKGACACMGGCACCTCTCTG | Exon7_R | CAGAGAGGTGCCKGTGTCMACAA |
| Exon8_F | CTGCCCACACTCGACTTCATCA | Exon8_R | TGATGAAGTCGAGTGTGGGCAG |
| Exon9_F | ACTGCATGGTGGGAATCCAG | Exon9_R | GAAGACATCWCCMAGGATCCAA |
| IntronA_F | GCAGCCCTTGAATGACAGGTAT | IntronA_R | CCTCCTTTTTCAGTATGAGTGCAA |
| IntronB_F | CTTGTGAGCTAACAGCCTGCC | IntronA_R2 | TGGCTCTCACTTACAATCTCCATG |
| IntronD_F | GCAAGGTTGAAGAATGAGCCTAG | IntronA_R3 | TTCCTCCATGACACCAACAAAGAC |
| IntronF_F | CCACAGGTGTCCTCTGCATGA | IntronE_R | TTCGGGTTTCATGTAGAGTGAG |
| IntronG_F | GGGTTAGAGCAAAATTACTGGAAC | 3’utr_R | CCAGAGAAGAGGCACAGATAGA |