Table 1.

Primer sequences used for RT-qPCR analyses of twelve dehydrins, three EARLY BUD-BREAK 1 orthologs and the FLOWERING LOCUS T-LIKE 2 transcripts and three reference genes. Sequences are listed in the 5′–3′ direction

Gene IDs	Accession no.a	Forward primer	Reverse primer	Product length (bp)
PaDHN 1	MA_95995g0010	GCGGCCTATGCGGCAAGAA	TCGACGAGCCCCGCCTTCTG	95
PaDHN 2.2	MA_187114g0010	CGCGGGCTGTTCGGTTTGTT	CAGCGGACGCAAGCAGAGGA	115
PaDHN 4.3	MA_8320994g0010	GCGGCGGACAGCATTCTTCG	TTGTTATCGTGGCCCGGAAGC	100
PaDHN 6	MA_747559g0010	TCCCGGAGGCCGGAACAAGT	CGAAAGCGACATGGAGAGGTAGCC	102
PaDHN 9	MA_2408574g0010	TCACGGTCAGCAGGGGCAAG	AACCGGAGCCGGAGCCATGT	101
PaDHN 13	MA_205576g0010	CCAGCTCAGAAGGCGGGGTTT	TGGCATCCAGGCAGCATCTC	116
PaDHN 23	MA_144878g0010	GACTACCAGGACCGCAGCCACA	GAGTCTGGCCTCCGGGAATCA	103
PaDHN 24	MA_12179g0010	CCCGGCTGTCTGGAATGCTC	CCGCCAAAACCCCTAGCAGAACA	90
PaDHN 35	MA_10428426g0010	GGACGAAGGAACGCAGGATGA	CTGGCATCCGGGCAGCTTCT	154
PaDHN 39	MA_86965g0010	CGAGGAGGATAAGGGCGGGAAT	TGCGTGGGTTGTAGCAGGTG	115
PaDHN 40	MA_10257300g0010	CGTGGCAGGAGCAGGCATCA	GAGCCGGAGCGCAAAGACCA	102
PaDHN 41	MA_10434136g0010	CCGCGAGAAGCCCGTCCATAC	CACCAGCAAGAACACCGGCTGA	98
PaEBB1.1	MA_27642g0010	TGGCTTCGACACAACTGATCCTACCA	TGTGGTTGATCTTGTGGCTGCTGT	114
PaEBB1.2	MA_30120g0010	CGCTCCTTCTTACTTTGGCTTCGAC	CGTGTTGCTGCTGCTGTAATTCTGG	119
PaEBB1.3	MA_77420g0010	CCACCTCGGGTTGTGGTGTTTGTC	GTCATAAGGCCAGTTGAGCCAATGC	88
PaFTL2 (Previously TFL1)	EF633467	ATGTTGGAGGAGACGACTTG	GTGTTGGATCGCTTGGACTA	81
Pa ACTIN	AY961918/MA_10427661g0030	TGAGCTCCCTGATGGGCAGGTGA	TGGATACCAGCAGCTTCCATCCCAAT	105
Pa αTub	X57980/ MA_93486g0010	GGCATACCGGCAGCTCTTC	AAGTTGTTGGCGGCGTCTT	66
Pa elF5α	AY961932/ MA_103714g0010	GCCGATGCGGGAGCTTCCAA	TGCAGGGCCTGGCCTTAATGACG	88

^aAccession no. based on Norway spruce genome sequence v.1. (http://congenie.org/)