Fig. 2.
Mapping of mitochondrial proteins into different classes. a Separation of the mitochondrial proteome into an integral membrane, a peripheral membrane, a soluble, and an ambiguous fraction. b Validation of map positions for several mitochondrial proteins by immunoblotting. c Map data correlation with known submitochondrial localization of components of the translocase of the inner membrane (TIM), the citrate cycle machinery, complex IV of the respiratory chain, and the Coenzyme Q biosynthesis apparatus5, 13, 24–26. Proteins in gray could not be mapped by LC-MS. d Distribution of the mitochondrial proteome into the indicated classes