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. 2017 Jun 7;49(9):1571–1585. doi: 10.1007/s00726-017-2449-7

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Purification of cathelicidin-PP from the skin of P. puerensis and MALDI–TOF MS. a The filtrate of the skin secretion of P. puerensis by 10 kDa cutoff was divided by a Wondasil C₁₈ RP-HPLC column. b The eluted peak (arrow in a) containing antimicrobial activity was further purified by C₁₈ RP-HPLC column. The purified cathelicidin-PP is indicated by an arrow. c MALDI-TOF mass spectrometry analysis of cathelicidin-PP