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. 2017 Aug 17;7:8608. doi: 10.1038/s41598-017-09111-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blots to identify the locations of PqiB and YebT in E. coli K-12 BW25113. The inner and outer membranes of the parental strain (WT) and the pqiAB (P), yebST (Y) and pqiAB yebST (PY) mutants were separated by sucrose gradients. The inner and outer membranes of each strain were taken as single fractions and separated by SDS-PAGE. Western blots using antibodies against known outer membrane marker TolC and inner membrane marker AcrB revealed that the separation of the inner and outer membranes was successful. Anti-PqiB and anti-YebT antibodies were used to identify the locations of PqiB and YebT. See Supplementary Fig. S9 for full western blots.