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. 2017 Aug 17;7:8608. doi: 10.1038/s41598-017-09111-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The phenotypes of MCE mutants on sulfobetaines and vancomycin. (A) Selected results from the BiOLOG analysis showing growth of the selected strains over time: Red = growth of the parent strain, green = growth of the mutant, yellow = overlay of growth between parent strain and mutant. LSB = lauryl sulfobetaine, CLQ = clioquinol, TET = tetracycline, PEN = penimepicycline, AZL = azlocillin. (B–F) Logarithmic dilutions of the parent and mutant strains on (B) LB agar, (C) 1% LSB, (D) 1% caprylyl sulfobetaine, (E) 1% myristyl sulfobetaine and (F) 300 μg/ml vancomycin. WT = parent strain, M = ΔmlaD, P = ΔpqiAB, Y = ΔyebST, MP = ΔmlaD ΔpqiAB, MY = ΔmlaD ΔyebST, PY = ΔpqiAB ΔyebST and MPY = ΔmlaD ΔpqiAB ΔyebST.