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. 2017 Aug 17;7:8546. doi: 10.1038/s41598-017-08668-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Promoter activity analysis of the bovine GPIHBP1 gene. There are four completely linked SNPs in the promoter region of GPIHBP1. As shown, fragments P1, P2, P3, and P4 contain one, two, three, and four SNPs sites, respectively. We constructed eight types of recombinant promoter vectors with respect to the four fragments and the mutant and wild-type haplotypes. Promoter activities were detected using a dual-luciferase reporter system. The data are expressed as the means and standard errors of three replicates.