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. 2017 Aug 17;8:273. doi: 10.1038/s41467-017-00386-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The nict-1 QTL controls a fitness trade-off and species dispersal on isopods. a Brood sizes of N2, CB4856, LJ1203 (nict-1 CB4856 > N2), and LJ1213 (nict-1 N2 > CB4856) are shown as Tukey box plots colored based on the nict-1 genotype. The horizontal line is the median, and the box denotes the 25th to 75th quantiles of the data. The vertical line represents the 1.5 interquartile range. All four strains were scored in 30 biological replicates, except for LJ1213 where only 29 biological replicates were scored. b The mean N2 nict-1 QTL allele frequencies before (origin) and after (destination) transmission competition assays are plotted. Error bars are standard deviations. c The experimental scheme of the transmission competition assay is shown. Transmission competition assay chambers contain two nematode culture plates. The “origin” plate contained a mixture of dauer animals from the CB4856 and LJ1213 (nict-1 N2 > CB4856) strains and was covered with medical gauze to facilitate nictation. The “destination” plate was placed 1–2 cm away with no nematodes on this plate. Terrestrial isopods were added to the chamber and allowed to roam freely for 24 h. Nematodes depend on the isopods for transfer to the “destination” plate from the “origin” plate because no transfer was observed without isopods presence. Eleven different biological replicates were scored