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. 2017 Aug 17;7:8547. doi: 10.1038/s41598-017-08950-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cdc42 and Rac1 activity during TNT formation in RAW/LR5 macrophages. (a) Representative images of time-lapse ratiometric imaging of RAW/LR5 macrophage cell line with inducible expression of Cdc42 biosensor in pre-existing TNTs (left panels) or TNT-precursors (right panels). Scale bars = 10 µm. (b) Representative images of time-lapse ratiometric imaging of RAW/LR5 macrophage cell line with inducible expression of Rac1 biosensor in pre-existing TNTs (left panels) or TNT-like precursors (right panels). Scale bars: Left = 10 µm, Right = 1.5 µm. (c) Quantitation of the frequency of Cdc42 activity (black bars) or Rac1 activity (grey bars) in the base and within pre-existing TNTs (left graph) or at the base, within and at the tip of growing TNT-like precursors (right graph). Data is represented as an average of at least 20 TNT structures. Error bars represent +/−SEM.