Figure 2.

FBN30 binds to P. berghei infected red blood cells and ookinetes. (a) Interaction between the recombinant FBN30 protein and P. berghei iRBC by ELISA. The lysates of P. berghei-infected red blood cells (iRBC) and uninfected mouse red blood cells (RBC) were used to determine the interaction between FBN30 and P. berghei parasites. OD₄₀₅ values and standard deviations were obtained from 3 replicates. The OD₄₀₅ value in P. berghei iRBC was 1.86 folds of the uninfected mouse RBC, and the difference is significant (p < 0.01). (b) Interaction between FBN30 protein and P. berghei parasites determined by indirect immunofluorescence assays (IFA). Images in the first row represent non-infected mouse RBCs. Images in the second row represent mouse P. berghei iRBCs and ookinetes. Images in the third row obtained by merging first and second rows to show the co-localization of FBN30 and parasites. Arrows show the location of P. berghei gametocytes. (c) FBN30 does not bind to P. berghei sporozoites as determined by IFA. Images in the first row are the control group by replacing FBN30 with equal amount of BSA in IFA assays. The first and second columns show P. berghei parasites (green color) and FBN30 (red color) respectively. Merging column one and two generated the third column.