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. 2005 Apr 11;102(16):5715–5720. doi: 10.1073/pnas.0409328102

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Copyright © 2005, The National Academy of Sciences

Freely available online through the PNAS open access option.

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Fig. 2. — Fluorescence time traces for single junction molecules. Solution conditions are as described in the text with a Mg²⁺ concentration of 50 mM. (A) A record of donor (green) and acceptor (red) fluorescence intensities (Upper) and calculated FRET efficiency (Lower) from a single molecule of J₁TGGGa as a function of time. The molecule interconverts between high and low FRET efficiency and exhibits two distinct dynamic behaviors. Arrows denote a transition between these forms at 3 s and the reverse at 7 s. (B) FRET efficiency as a function of time for J₁TGGG. (C) FRET efficiency as a function of time for J₁TTGT.