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. 2017 Aug 17;7:8574. doi: 10.1038/s41598-017-08795-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Brain-derived neurotrophic factor (BDNF) levels in prefrontal cortex of adolescent and adult male Swiss mice as a function of rearing condition (standard [control] conditions and environmental enrichment) and treatment (4, every-other-day administrations of 0.0 [vehicle control] or 2.0 g/kg ethanol). The adolescent group treated with ethanol and exposed to standard (control) rearing conditions (first black bar on the left) exhibited significantly less BDNF levels than (A) any of the other groups (adolescents or adults) reared under standard housing conditions and treated with vehicle [these significant differences are indicated by the pound (#) and currency ($) signs, respectively], (B) the adults treated with ethanol and exposed to standard (control) rearing conditions [a significant difference indicated by the ampersand (&) sign] and C) adolescents reared under environmental enrichment conditions and treated with ethanol. The latter significant difference is are indicated by the asterisk (*) sign. Values express mean ± SEM.