Table 1. Distribution of GnRH subtype immunopositive neurons in the brain of WT and EGFP+/GnRH1 and double-label GnRH neurons in transgenic rats.
| GnRH antibody
|
Antibody
|
%CR with GnRH1
|
Soma/fibers
|
Septal-preoptic hypothalamus
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Concentration | Code | TT-SHi | MS-DB | MPOA | LS | OVLT | ME | Pe | SO | VMHvl | Am | HB | PVP | CA1 | MM | CG | IP | IR cells | IF cells | EGFP+ cells | DL cells | % DL | ||
| GnRH1-EGFP+ | — | — | — | +/+ | +/+ | +/+ | -/+ | -/+ | -/+ | +/+ | +/+ | +/+ | -/+ | +/+ | -/+ | -/+ | +/+ | -/+ | -/+ | — | — | 901 ± 25 | — | — |
| GnRH1 | 1:2,500 | LRH13 | 100 | +/+ | +/+ | +/+ | -/- | -/+ | -/+ | +/+ | +/+ | +/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/+ | 962 ± 26 | 1044 ± 51 | 908 ± 49 | 860 ± 42 | 82 ± 1 |
| GnRH1 | 1:8,000 | 635.5 | NA | +/+ | +/+ | +/+ | -/- | -/+ | -/+ | -/+ | +/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/+ | 964 ± 88 | ||||
| GnRH1 | 1:2,000 | AB1567 | 100 | -/+ | +/+ | +/+ | -/- | -/+ | -/+ | -/- | -/+ | -/- | -/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/+ | 124 ± 28 | ||||
| GnRH2 | 1:4,000 | aCII6 | 0.01 | -/- | +/+ | +/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/- | +/+ | -/+ | -/+ | -/- | -/+ | -/+ | -/+ | 456 ± 84 | 413 ± 74 | 861 ± 41 | 288 ± 82 | 70 ± 2 |
| GnRH2 | 1:3,000 | 1458 | 2.5 | +/+ | +/+ | +/+ | -/- | -/+ | -/+ | +/+ | +/+ | -/+ | -/+ | -/+ | -/+ | -/+ | -/- | -/+ | -/+ | 472 ± 8 | ||||
| GnRH2 | 1:3,000 | ISP II | NA | -/- | -/- | +/+ | -/- | -/+ | -/+ | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | 84 ± 20 | ||||
| GnRH3 | 1:11,500 | Lot. 2 | 0.01 | +/+ | +/+ | +/+ | -/- | -/+ | -/+ | -/+ | +/+ | +/+ | -/+ | -/+ | -/+ | -/- | -/+ | -/+ | -/+ | 516 ± 80 | 670 ± 76 | 972 ± 52 | 406 ± 24 | 60 ± 7 |
| GnRH3 | 1:3,500 | GF6 | 100 | +/+ | +/+ | +/+ | -/- | -/+ | -/+ | +/+ | +/+ | +/+ | -/+ | -/+ | -/+ | -/- | -/+ | -/+ | -/- | 616 ± 18 | ||||
| Seabream | 1:5,000 | ISP1 | 0.01 | -/- | +/+ | +/+ | -/- | -/+ | -/+ | -/- | -/+ | -/+ | -/+ | -/+ | -/- | -/- | -/+ | -/+ | -/- | 45 ± 8 | ||||
CR, cross reactivity; Shi, septohippocampal nucleus; MS-DB, medial septum/diagonal band of Broca; LS, lateral septum; Pe, periventricular hypothalamic nucleus; SO, supraoptic nucleus; VMHvl, ventrolateral part of ventromedial hypothalamus; Am, medial amygdaloid nucleus; PVP, periventricular thalamic nucleus; CA1, CA1 field of the hippocampus; CG, midbrain central gray; IP, interpeduncular nucleus; IR, peroxidase immunoreactivity; IF, immunofluorescence; DL, double-label; NA, not available. % DL, DL/IF × 100. +/- indicates presence/absence of cell soma (Left) and fibers (Right). GnRH immunoreactive and EGFP+ cell numbers are mean ± SEM from three to four animals per antibody and four EGFP+ animals. The sources (represented by code) of the antisera are given in Materials and Methods.