Figure 2.

Efficiency of bacterial transformation using shockwaves and its comparison with electroporation. (a) E.coli, (b) P.aeruginosa, (c) Salmonella Typhimurium, (d) H. pylori, (e) Mycobacterium smegmatis and (f) Mycobacterium tuberculosis were transformed with relevant plasmids mainly by heat shock, electroporation and shock waves. After transformation LB broth was added to the cells, incubated at 37 °C for 1 h before plating. For E.coli, P.aeruginosa, Salmonella and H.pylori the shocktube fill pressure was varied. For Mycobacterial transformations, the shocktube fill pressure was maintained at 10 bar and the number of consecutive shockwave exposures were varied to achieve high efficiency transformation. Post incubation, CFU were counted and transformation efficiency was calculated. Statistical significance was defined as follows (*P < 0.05, **P < 0.005, ***P < 0.0005) (Student’s t test).