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. 2017 Aug 17;7:8509. doi: 10.1038/s41598-017-08226-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of biocompatibility of different coaxial scaffolds. (A–D) Representative images of live-dead staining of rMSCs after 1 day culture on different scaffolds SF/PLCL-PEO (A), SF/PLCLPEO-FGF2 (B), SF-CTGF/PLCL-PEO (C) and SF-CTGF/PLCL-PEO-FGF2 (D). All cells were counterstained with NucBlue live reagent, nuclei of dead cells were counterstained with NucGreen dead reagent. (E) The quantified data from (A–D), at least 200 cells were counted for each group. (F) Analysis of cytotoxicity of different scaffolds to rMSCs after 1 day culture by LDH assay. (G) Analysis of rMSCs proliferation on different scaffolds after a 14-day incubation by CCK assay. (One-way repeated-measure Analysis of Variance test (ANOVA) were used to compare multiple groups. * and *** stand for significant difference between samples on scaffolds and samples on tisue culture plastic (TCP) at same time point with p < 0.05 and p < 0.001, respectively; # and ## stand for significant difference among different scaffolds at same time point with p < 0.05 and p < 0.01, respectively).