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. 2017 Aug 17;7:8594. doi: 10.1038/s41598-017-08806-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of PSY1-circ1 in Ailsa Craig. (a) Fruits of different PSY1-circ1 overexpression tomato lines. OE3-OE28 stand for transgenic lines. Vector control stands for Ailsa Craig (AC) plants transformed with the empty vector pCAMBIA1301. (b) Lycopene and β-carotene contents in transgenic tomato fruits at breaker +10 stage. White, black and gray bars stand for control, transgenic lines with red fruits and yellow fruits respectively. The data with error bars were expressed as mean ± SEM (Standard Error of the Mean, n = 4), asterisks indicate statistically significant difference (with respect to control; *p value < 0.05; **p value < 0.01; two-way ANOVA followed by LSD (Least Significant Difference) test). (c) Expression profile of PSY1-circ1, linear RNAs generated by the overexpression vector, and PSY1 gene in transgenic tomato fruits at different ripening stages. The data are normalized to Actin and presented as means ± SD (n = 5).