FIG 3.

Functional analysis of UreH. (A) Alignment of UreH from B. paralicheniformis ATCC 9945a with the nickel transport protein from L. thermophila and with Bacillus sp. strain TB-90 UreH. (B) Topology of nickel/cobalt transporters (NiCoTs) and UreH from B. paralicheniformis ATCC 9945a. The signature sequence HX₄DH in TMD II of the NiCoT and the corresponding sequence HAIEPDH in UreH are shown above the diagrams. (C) SDS-PAGE analysis of UreH expression. Lane M, protein molecular weight standard; lane 1, BL21(DE3)/pRSFDuet-1 cell lysis supernatant; lane 2, BL21(DE3)/pRSFDuet-ureH cell lysis supernatant; lane 3, purified UreH. (D) Intracellular metal ion contents of recombinant strains. After induction for 4 or 8 h, strains were washed and lysed, and the soluble extracts were used for the analysis of metal ion contents by atomic absorption spectrometry.