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Evaluation of urea and urethane degradation by BpUrease (Fe3+) in rice wine The purified BpUrease (Fe3+) (at a final titer of 2 or 6 U/ml) was added to a wine sample (pH 4.5; 15% [vol/vol] ethanol), and the reaction system was incubated at 37°C for 2 days.
