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. 2017 Aug 17;83(17):e01258-17. doi: 10.1128/AEM.01258-17

TABLE 1.

Kinetic parameters of BpUrease

Substrate and enzyme Sp act (U · mg−1) Km (mM) Vmax (μmol · mg−1 · min−1) kcat (s−1) kcat/Km (s−1 · M−1)
Urethane
    BpUrease (Ni2+) 23.1 ± 0.4 1,018 ± 58 118 ± 5 433 ± 22 425 ± 19
    BpUrease (Fe3+) 2.5 ± 0.2 958 ± 52 15.2 ± 1.1 56 ± 3 58 ± 3.9
Urea
    BpUrease (Ni2+) 603 ± 13 23.7 ± 1.5 769 ± 39 2,820 ± 135 (1.2 ± 0.1)E + 05
    BpUrease (Fe3+) 54.6 ± 2.1 28.2 ± 1.7 73.5 ± 5.3 270 ± 14 9,557 ± 478