TABLE 1.
Substrate and enzyme | Sp act (U · mg−1) | Km (mM) | Vmax (μmol · mg−1 · min−1) | kcat (s−1) | kcat/Km (s−1 · M−1) |
---|---|---|---|---|---|
Urethane | |||||
BpUrease (Ni2+) | 23.1 ± 0.4 | 1,018 ± 58 | 118 ± 5 | 433 ± 22 | 425 ± 19 |
BpUrease (Fe3+) | 2.5 ± 0.2 | 958 ± 52 | 15.2 ± 1.1 | 56 ± 3 | 58 ± 3.9 |
Urea | |||||
BpUrease (Ni2+) | 603 ± 13 | 23.7 ± 1.5 | 769 ± 39 | 2,820 ± 135 | (1.2 ± 0.1)E + 05 |
BpUrease (Fe3+) | 54.6 ± 2.1 | 28.2 ± 1.7 | 73.5 ± 5.3 | 270 ± 14 | 9,557 ± 478 |