TABLE 2.

Strains and plasmids used in this study

Strain or plasmid	Description	Source
Strains
Bacillus paralicheniformis ATCC 9945a	Used for cloning the iron-containing urease	CGMCC
Providencia sp. LBBE 918	Used for cloning the nickel-containing urease	CCTCC
Escherichia coli	Used for plasmid screening and propagation	Invitrogen
JM109
BL21(DE3)	Used for heterologous protein expression	Novagen
BL21(DE3)/pRSFDuet-Bpure-1	BL21(DE3) derivative harboring plasmid pRSFDuet-Bpure-1	This work
BL21(DE3)/pRSFDuet-Bpure-2	BL21(DE3) derivative harboring plasmid pRSFDuet-Bpure-2	This work
BL21(DE3)/pRSFDuet-Bpure-ΔH	BL21(DE3) derivative harboring plasmid pRSFDuet-Bpure-ΔH	This work
BL21(DE3)/pRSFDuet-ureH	BL21(DE3) derivative harboring plasmid pRSFDuet-ureH	This work
BL21(DE3)/pRSFDuet-Psure-1	BL21(DE3) derivative harboring plasmid pRSFDuet-Psure-1	This work
BL21(DE3)/pRSFDuet-Psure-2	BL21(DE3) derivative harboring plasmid pRSFDuet-Psure-2	This work
Plasmids
pRSFDuet-1	Kanr; T7 promoter	Novagen
pRSFDuet-Bpure-1	Overexpression of BpUrease gene cluster in E. coli	This work
pRSFDuet-Bpure-2	Overexpression of BpUrease gene cluster in which a Strep tag was added to the amino terminus of the C subunit in E. coli	This work
pRSFDuet-Bpure-ΔH	Overexpression of BpUrease gene cluster in which ureH was knocked out from the gene cluster in E. coli	This work
pRSFDuet-ureH	Overexpression of ureH in E. coli	This work
pRSFDuet-Psure-1	Overexpression of PsUrease gene cluster in E. coli	This work
pRSFDuet-Psure-2	Overexpression of PsUrease gene cluster in which a Strep tag was added to the amino terminus of the C subunit in E. coli	This work