FIG 5.

Biosynthetic gene clusters and LanA alignments of type 2 lanthipeptides with dual LanM enzymes identified in non-aureus staphylococci isolated from milk of Canadian dairy cows. (A) Biosynthetic gene clusters of identified dual precursor type 2 lanthipeptides, with inhibiting NAS isolates in boldface. (B-1) Multiple-sequence alignments of LanA1 genes identified in type 2 lanthipeptide clusters and known bacteriocins lacticin 3147 A1 (accession number O87236), lichenicidin A1 (accession number P86475), and staphylococcin C55 A1 (accession number Q9S4D3). (B-2) Multiple-sequence alignments of LanA2 genes identified in type 2 lanthipeptide clusters and known bacteriocins lacticin 3147 A2 (accession number O87237), lichenicidin A2 (accession number P86476), and staphylococcin C55 A2 (accession number Q9S4D2).