TABLE 1.
Primers and thermal cycling profiles for real-time PCR analysisa
| Targeted gene | Oligonucleotide sequence (5′–3′) | Reference |
|---|---|---|
| Reg3β | Forward: GGCTTCATTCTTGTCCTCCA | 43 |
| Reverse: TCCACCTCCATTGGGTTCT | ||
| Reg3γ | Forward: AAGCTTCCTTCCTGTCCTCC | 43 |
| Reverse: TCCACCTCTGTTGGGTTCAT | ||
| MUC2 | Forward: GCTGACGAGTGGTTGGTGAATG | 44 |
| Reverse: GATGAGGTGGCAGACAGGAGAC | ||
| IL-22 | Forward: TTGAGGTGTCCAACTTCCAGCA | 45 |
| Reverse: AGCCGGACGTCTGTGTTGTTA | ||
| GAPDH | Forward: ATTGTCAGCAATGCATCCTG | 13 |
| Reverse: ATGGACTGTGGTCATGAGCC |
a
The annealing temperature used for all of the genes was 60°C.