FIG 5.
ADP1-ISx exhibits increased transformability and reduced autolysis. (A) Transformation frequencies measured as transformants per CFU under normal growth conditions in LB medium for PCR products containing the tdk-Kanr cassette (into site 2) or a spectinomycin resistance marker (into site 4). (B) CFU measured by plating dilutions of cultures on LB agar after saturating growth. (C) Reduced settling behavior of ADP1-ISx compared to ADP1 in saturated cultures left at room temperature for 48 h after growth. (D) Accumulation of extracellular DNA over time in the supernatants of MS cultures grown under standard conditions. (E) Fraction of cells staining as dead in LB cultures after 24 h of growth. The error bars are 95% confidence intervals.