TABLE 1.

Primers used in this study and results from Primer Prospector in silico analysis

rDNA	Primer	Sequence (5′ → 3′)	Amplicon length (bp)	Reference no.	Non-3′ mismatcha	3′ mismatcha	Non-3′ gapsa	3′ gapsa	Weighted scorea
SSU	18Sf	ATTCCAKCTCCAAKAGCG	436	17	0.137	0.055	0.002	0.000	0.156
	18Sr	GATNAGATACCRTCGTAGTC		17	0.445	0.012	0.009	0.000	0.245
LSU	26Sf	GCATATCAATAAGCGGAGGAAAAG	579	29	2.977	0.339	0.018	0.000	1.720
	26Sr	CCGTCTTGAAACACGGACCG		29	1.556	0.158	0.012	0.001	0.904
ITS1-2	ITS1f	TCCGTAGGTGAACCTGCGG	Variable	23	3.503	1.024	0.059	0.002	3.050
	ITS2r	GCATCGATGAAGAACGCAGC		23	0.527	0.164	0.021	0.001	0.593

^aWeighted score = (non-3′ mismatches × 0.4) + 3′ mismatches + non-3′ gaps + (3′ gaps × 3.0) + final 3′ base mismatch × 3.0).