FIG 4.

Biofilm formation. The S. mutans wild-type strain (UA159) and its rgpG (TW322), brpA (TW14D), psr (TW251), rpgG brpA (TW340), rgpG psr (TW341), and rgpG brpA psr (TW343) mutants were grown in BM containing glucose, sucrose, and glucose plus sucrose. Biofilms were grown in 96-well plates and measured using a spectrophotometer following crystal violet staining. ANOVA and Tukey's pairwise comparison were used to analyze the differences between different strains. The data presented here represent the average (±SD shown by error bars) from more than three separate sets of experiments with cultures grown in BM plus glucose and sucrose. #, P < 0.001 versus UA159 and TW343 and <0.05 versus TW14D, TW251, TW340, and TW341; *, P < 0.001 versus UA159 and TW343 and <0.05 versus TW322; **, P < 0.001 versus all others.